Production of β‐glucosidase (cellobiase) by Curvularia sp.

作者: U. C. Banerjee

DOI: 10.1111/J.1472-765X.1990.TB01332.X

关键词: Beta-glucosidaseBiologyBiochemistryExtracellularCellulaseEnzyme assayEnzymeFood scienceYeast extractMicroorganismCurvularia

摘要: A Curvularia sp. isolated from soil was found to produce extracellular β-glucosidase activity when grown in yeast extract, peptone, carboxymethylcellulose (YPC) medium. An initial medium pH of 6·5 and cultivation temperature 30°C were be most suitable for high enzyme productivity. The optima the 4·0 70°C, respectively. Under these conditions, exhibited a Km (0-nitrophenyl-β-d-glucoside) value 0.20 mmol/l. Several divalent metal ions inhibited at concentration. EDTA. also activity.

参考文章(8)
Wesley E. Workman, Donal F. Day, Purification and Properties of β-Glucosidase from Aspergillus terreus Applied and Environmental Microbiology. ,vol. 44, pp. 1289- 1295 ,(1982) , 10.1128/AEM.44.6.1289-1295.1982
Chie KOHCHI, Mitsunori HAYASHI, Shiro NAGAI, Purification and Properties of β-Glucosidase from Candida pelliculosa var. acetaetherius Agricultural and biological chemistry. ,vol. 49, pp. 779- 784 ,(1985) , 10.1271/BBB1961.49.779
Gentaro OKADA, Purification and Properties of a Cellulase from Aspergillus niger Agricultural and biological chemistry. ,vol. 49, pp. 1257- 1265 ,(1985) , 10.1271/BBB1961.49.1257
A.S.L. Hu, R. Epstein, H.O. Halvorson, R.M. Bock, Yeast β-glucosidase: Comparison of the physical-chemical properties of purified constitutive and inducible enzyme☆ Archives of Biochemistry and Biophysics. ,vol. 91, pp. 210- 218 ,(1960) , 10.1016/0003-9861(60)90492-6
Gail Lorenz Miller, Protein determination for large numbers of samples. Analytical Chemistry. ,vol. 31, pp. 964- 964 ,(1959) , 10.1021/AC60149A611
Jonathan Woodward, Alan Wiseman, Fungal and other β-d-glucosidases — Their properties and applications Enzyme and Microbial Technology. ,vol. 4, pp. 73- 79 ,(1982) , 10.1016/0141-0229(82)90084-9
ChristineS. Evans, Properties of the β-d-glucosidase (cellobiase) from the wood-rotting fungus, Coriolus versicolor Applied Microbiology and Biotechnology. ,vol. 22, pp. 128- 131 ,(1985) , 10.1007/BF00250032