In vitro reactivity of mast cells in urticaria pigmentosa skin
作者： E. Brzezińska-Błaszczyk , Anna Zalewska
关键词: Immunology 、 Medicine 、 Neurogenic inflammation 、 Enzyme 、 Mast (sailing) 、 Mast cell 、 In vitro 、 Substance P 、 Urticaria pigmentosa 、 Histamine
摘要: The aim of our study was to evaluate the sensitivity skin mast cells from urticaria pigmentosa (UP) patients substance P (SP), tumor necrosis factor α (TNF-α) and anti-IgE, compare these with that healthy human donors. Mast for in vitro functional studies were obtained using an enzymatic dispersion technique biopsies (from 11 UP donors), reactivity estimated on basis histamine release. Our observations indicated had similar sensitivities challenge TNF-α at a concentration 10–7M (16.4% vs 15.2%) anti-IgE dilution 1:100 (41.0% 37.0%). However, showed considerably higher SP 10–4M than (20.0% 6.8%), difference statistically significant (P < 0.001). also demonstrated significantly spontaneous release (32.1% 12.4%, findings indicating cell might suggest mechanisms involving neurogenic inflammation could contribute course this disease.
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