Quantitative analysis of contact sites between mast cells and sensory nerves in cutaneous psoriasis and lichen planus based on a histochemical double staining technique.

摘要: The aim of the present study was to test further our previous hypothesis that inflammatory reaction in psoriasis is neurogenic. For this purpose, contact sites between mast cells and sensory nerves were morphometrically analysed basement membrane zone, papillary dermis three dermal zones lesional/non-lesional psoriatic lichen planus skin as well healthy control skin. analyses made on sections stained with a histochemical double stain developed for study. With stain, active cell tryptase blue enzyme histochemically, black using specific monoclonal anti-neurofilament antibodies immunogold. In lesions, both cell--nerve contacts markedly more frequent zone when compared corresponding areas other groups. Mast numbers increased lesional symptom-free planus, but no increase found contacts. Increased indicate elements exist neurogenic inflammation lesions. These are not due extensive only, because they observed