Mast cell tryptase and chymase in developing and mature psoriatic lesions.
作者： I. T. Harvima , A. Naukkarinen , K. Paukkonen , R. J. Harvima , M. -L. Aalto
DOI: 10.1007/BF00372007
关键词: Psoriasis 、 Mast cell 、 Papillary dermis 、 Tryptase 、 Pathology 、 Antibody 、 Chymase 、 Biology 、 Immunohistochemistry 、 Dermis
摘要: The number and distribution of mast cells in non-lesional lesional skin samples from 13 psoriatic patients were analyzed enzyme- immunohistochemically. Mast cell tryptase was stained with the sensitive substrate Z-Gly-Pro-Arg-4-methoxy-2-naphthylamide, chymase Suc-Val-Pro-Phe-MNA monoclonal B7 anti-chymase antibody. In addition, healthy-looking 27 tape-stripped resulting induction Kobner response 9 patients. Sequential biopsies taken before after (7, 14 21 days) tape-stripping, both enzyme-histochemically. skin, 70 +/- 24% (mean SD) contained enzyme activity, 78 18% immunoreactivity. About 10% chymase-immunoreactive lacked activity. tryptase-positive increased throughout dermis but especially beneath epidermis. Chymase immunoreactivity paralleled whereas activity strongly diminished terms numbers staining intensity papillary dermis. apparent inactivation may be due to action inhibitors, alpha 1-antitrypsin 1-antichymotrypsin, localized immunohistochemically skin. developing lesion, displaying already 27-38% decreased upper on day 7 along first clinical signs psoriasis.(ABSTRACT TRUNCATED AT 250 WORDS)
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sci-hub.se 参考文章(44)
G.H. Caughey, E.H. Zerweck, P. Vanderslice, Structure, chromosomal assignment, and deduced amino acid sequence of a human gene for mast cell chymase Journal of Biological Chemistry. ,vol. 266, pp. 12956- 12963 ,(1991) , 10.1016/S0021-9258(18)98788-0
N M Schechter, J E Fräki, J C Geesin, G S Lazarus, Human skin chymotryptic proteinase. Isolation and relation to cathepsin g and rat mast cell proteinase I. Journal of Biological Chemistry. ,vol. 258, pp. 2973- 2978 ,(1983) , 10.1016/S0021-9258(18)32816-3
N M Schechter, J L Sprows, O L Schoenberger, G S Lazarus, B S Cooperman, H Rubin, Reaction of human skin chymotrypsin-like proteinase chymase with plasma proteinase inhibitors. Journal of Biological Chemistry. ,vol. 264, pp. 21308- 21315 ,(1989) , 10.1016/S0021-9258(19)30080-8
AM Irani, SM Goldstein, BU Wintroub, T Bradford, LB Schwartz, None, Human mast cell carboxypeptidase : selective localization to MCTC cells Journal of Immunology. ,vol. 147, pp. 247- 253 ,(1991)
N. M. Schechter, A.-M. A. Irani, B. Wintroub, L. B. Schwartz, J. Abernethy, J. L. Sprows, Identification of a cathepsin G-like proteinase in the MCTC type of human mast cell. Journal of Immunology. ,vol. 145, pp. 2652- 2661 ,(1990)
Jorma E. Fräki, Väinö K. Hopsu-Havu, Human skin proteases Archives of Dermatological Research. ,vol. 256, pp. 113- 126 ,(1976) , 10.1007/BF00567358
Craig Hammerberg, James T. Elder, James T. Elder, Gary Fisher, Kevin D. Cooper, Lawrence S. Chan, John J. Voorhees, Ole Baadsgaard, Daniel N. Sauder, IL-1 activity is reduced in psoriatic skin. Decreased IL-1 alpha and increased nonfunctional IL-1 beta. Journal of Immunology. ,vol. 144, pp. 4593- 4603 ,(1990)
S Sayama, R V Iozzo, G S Lazarus, N M Schechter, Human skin chymotrypsin-like proteinase chymase. Subcellular localization to mast cell granules and interaction with heparin and other glycosaminoglycans. Journal of Biological Chemistry. ,vol. 262, pp. 6808- 6815 ,(1987) , 10.1016/S0021-9258(18)48317-2
G H Caughey, N F Viro, J A Nadel, F Leidig, Substance P and vasoactive intestinal peptide degradation by mast cell tryptase and chymase. Journal of Pharmacology and Experimental Therapeutics. ,vol. 244, pp. 133- 137 ,(1988)
Masashi Akiyama, Yonosuke Watanabe, Takeji Nishikawa, Immunohistochemical Characterization of Human Cutaneous Mast Cells in Urticaria Pigmentosa (Cutaneous Mastocytosis) Pathology International. ,vol. 41, pp. 344- 349 ,(1991) , 10.1111/J.1440-1827.1991.TB01657.X