Protection of chickens against highly lethal H5N1 and H7N1 avian influenza viruses with a recombinant fowlpox virus co-expressing H5 haemagglutinin and N1 neuraminidase genes

摘要: Inactivated whole avian influenza virus (AIV) vaccine provides protection against homologous haemagglutinin (HA) subtype virus, but poor a heterologous HA virus. Moreover, it induces chickens to produce antibodies cross-reactive antigens, especially nucleoprotein, which is limits AIV serological surveillance. In this study, recombinant fowlpox co-expressing (H5 subtype) and NA (NI subtype)genes of was evaluated for its ability protect intramuscular challenge with lethal dose highly pathogenic (HP) AIV. Susceptible were also vaccinated by wing-web puncture the parent Following 4 weeks later HPAIV, all protected, while either unaltered or unvaccinated controls experienced 100% mortality following challenge. This accompanied high levels specific antibody respective components vaccine. The above results showed that rFPV-HA-NA could be potential replace current inactivated vaccines preventing AI.