Leukotrienes C4 and D4 stimulate human endothelial cells to synthesize platelet-activating factor and bind neutrophils.

摘要: Abstract Human endothelial cells in culture produced platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with leukotriene C4 or D4 (LTC4 LTD4). Other arachidonate metabolites did not induce the synthesis of PAF. Accumulation PAF was a prolonged response maximal accumulation after 10-20 min stimulation. Half this amount remained 90 The synthesized by associated these cells. LTC4 and LTD4 also induced adherence human neutrophils (polymorphonuclear leukocytes; PMNs) to cell monolayer. Adhesion an cell-mediated process because PMNs adhered monolayers that had been washed prior PMN addition, neither nor absence time course adhesion paralleled cells, exogenously added adherence. specifically desensitized showed only 39% agonist-stimulated control PMNs. We conclude retained LTC4- LTD4-stimulated may previously unstimulated This be relevant inflammation, thrombosis, mechanisms vascular injury, including atherosclerosis.