Protein microarray technology

作者: Dieter Stoll

DOI: 10.2741/STOLL

关键词: Plasma protein bindingLigand binding assayChemistryChemical compound microarrayRIP-ChipProtein microarrayMicroarray databasesDNAComputational biologyProteomicsGeneral Biochemistry, Genetics and Molecular BiologyGeneral Immunology and MicrobiologyGeneral Medicine

摘要: This review summarizes the major activities in field of protein microarray technology. A short summary theoretical concepts miniaturized ligand binding assays explains why such microspot represent most sensitive approaches for capture-target assays. The main focus this is centered on applications using and parallelized which rely product formation between immobilized capture molecules their corresponding target are present sample. These types useful tools identification, quantification affinity studies. Protein identification have a great potential diagnostics proteomics where many different markers complex samples to be analyzed parallel. can used analyze interactions proteins as antibodies, receptors or enzymes with other proteins, peptides, low molecular weight compounds, oligosaccharides DNA. Different microarray-based huge diagnostic proteomic will discussed.

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