The origins of eukaryotic-like proteins in Legionella pneumophila

摘要: Legionella pneumophila, the causative agent of Legionnaires' disease, is known to be an intracellular pathogen multiple species protozoa and assumed have co-evolved with these organisms for millions years. Genome sequencing L. pneumophila strains has revealed abundance eukaryotic-like proteins (ELPs). Here, we study evolution ELPs, in order investigate their origin. Thirty-four new ELPs were identified, based on a higher similarity eukaryotic than bacterial ones. Phylogenetic analyses demonstrated that both lateral gene transfer from hosts genes became by gradual adaptation milieu or fragment acquisition, contributed existing repertoire which comprise over 3% putative proteome strains. A PCR survey 72 showed most conserved nearly all strains, indicating they are likely play important roles this species. Genes different evolutionary origin distinct patterns selection, as reflected ratio synonymous vs. mutations. One ELP common several Legionella, but outside genus homologs only Acanthamoeba polyphaga mimivirus, exchange involving viruses pathogens may also contribute virulence either groups organisms. Information selection status was combined novel approach predict type IV secretion system effectors represent promising targets future study.