Cell directional migration and oriented division on three-dimensional laser-induced periodic surface structures on polystyrene.

作者: Xuefeng Wang , Christian A. Ohlin , Qinghua Lu , Jun Hu

DOI: 10.1016/J.BIOMATERIALS.2007.12.047

关键词: CellPolystyreneEpithelial carcinomaNanotechnologyCell divisionMaterials scienceCell migrationBiophysicsFocal adhesionLaserExtracellular matrix

摘要: The extracellular matrix in animal tissues usually provides a three-dimensional structural support to cells addition performing various other important functions. In the present study, wavy submicrometer laser-irradiated periodic surface structures (LIPSS) were produced on smooth polystyrene film by polarized laser irradiation with wavelength of 266 nm. Rat C6 glioma exhibited directional migration and oriented division polystyrene, which was parallel direction LIPSS. However, rat moved three-step invasion cycle, faster speed than that polystyrene. addition, focal adhesions examined immunostaining adhesion kinase human epithelial carcinoma HeLa punctuated whereas dash-like We hypothesized LIPSS acted as an anisotropic persistent mechanical stimulus guide cell spreading, through adhesions.

参考文章(44)
Manuel Théry, Andrea Jiménez-Dalmaroni, Victor Racine, Michel Bornens, Frank Jülicher, Experimental and theoretical study of mitotic spindle orientation Nature. ,vol. 447, pp. 493- 496 ,(2007) , 10.1038/NATURE05786
Hiroyuki Hiraoka, Mariana Sendova, Laser‐induced sub‐half‐micrometer periodic structure on polymer surfaces Applied Physics Letters. ,vol. 64, pp. 563- 565 ,(1994) , 10.1063/1.111104
Jamil A. Matthews, Gary E. Wnek, David G. Simpson, Gary L. Bowlin, Electrospinning of collagen nanofibers. Biomacromolecules. ,vol. 3, pp. 232- 238 ,(2002) , 10.1021/BM015533U
Manuel Théry, Victor Racine, Anne Pépin, Matthieu Piel, Yong Chen, Jean-Baptiste Sibarita, Michel Bornens, The extracellular matrix guides the orientation of the cell division axis Nature Cell Biology. ,vol. 7, pp. 947- 953 ,(2005) , 10.1038/NCB1307
Matthew J Dalby, Mathis O Riehle, Stephen J Yarwood, Chris D.W Wilkinson, Adam S.G Curtis, Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography. Experimental Cell Research. ,vol. 284, pp. 274- 282 ,(2003) , 10.1016/S0014-4827(02)00053-8
Ki-Hwan Kim, Jun-Sik Cho, Doo-Jin Choi, Seok-Keun Koh, Hydrophilic group formation and cell culturing on polystyrene Petri-dish modified by ion-assisted reaction Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms. ,vol. 175, pp. 542- 547 ,(2001) , 10.1016/S0168-583X(00)00650-9
Satyajit K. Mitra, Daniel A. Hanson, David D. Schlaepfer, Focal adhesion kinase: in command and control of cell motility Nature Reviews Molecular Cell Biology. ,vol. 6, pp. 56- 68 ,(2005) , 10.1038/NRM1549
Manuel Théry, Michel Bornens, Cell shape and cell division. Current Opinion in Cell Biology. ,vol. 18, pp. 648- 657 ,(2006) , 10.1016/J.CEB.2006.10.001
Christopher S Chen, Milan Mrksich, Sui Huang, George M Whitesides, Donald E Ingber, None, Geometric Control of Cell Life and Death Science. ,vol. 276, pp. 1425- 1428 ,(1997) , 10.1126/SCIENCE.276.5317.1425
Stephen Doxsey, Wendy Zimmerman, Keith Mikule, Centrosome control of the cell cycle Trends in Cell Biology. ,vol. 15, pp. 303- 311 ,(2005) , 10.1016/J.TCB.2005.04.008