Safranine as a probe of the mitochondrial membrane potential

摘要: One of the most central postulates chemiosmotic hypothesis oxidative phosphorylation is existence a large electrochemical proton gradient (protonmotive force) across mitochondrial membrane [ 1,2] . This gradient, which composed potential and pH differential, postulated to be obligatory link between respiration in process phosphorylation. The has been suggested major component under conditions, measurements this are therefore essential for understanding mechanism ion transport. mainly estimated from distribution K’ or Rb’ presence ionophore valinomycin [3-g]. method given fairly consistent results potentials range 130190 mV (positive polarity extramitochondrially) have generally reported coupled mitochondria supplemented with either substrate ATP. differences values by different workers may part attributed use matrix volume. kind, requires partially artificial experimental makes confirmation other independent methods desirable. prompted several search suitable spectroscopic probes. fluorescent probe MC V used Chance collaborators [9,10] as indicator mitochondria, Laris et al. 111 employed cyanine dye