Conservation strategies for jerangau merah (Boesenbergia stenophylla) using DNA profiling and micropropagation
作者： Franklin Ragai Kundat , Thohirah Lee Abdullah , Nur Ashikin Psyquay Abdullah , Ghizan Saleh , Aicher Joseph Toyat
DOI:
关键词: Boesenbergia 、 Microsatellite 、 Micropropagation 、 DNA profiling 、 Horticulture 、 Genetic variation 、 Genetic marker 、 Biology 、 RAPD 、 Shoot 、 Botany
摘要: Jerangau merah (Boesenbergia stenophylla) is highly endemic to the highland of Borneo. Their medicinal value attracts many plant collectors which raise up concern on their population size. A study was carried out establish conservation approaches for this species. The objectives are determine genetic variations among accession from Bario and develop in vitro culturing protocol productions seedlings. Genetic variation studies were done using simple sequence repeats (SSR) random amplified polymorphic DNA markers (RAPD). Micropropagation shoot tips BAP NAA growth regulator supplemented MS media. SSR RAPD marker show no three sub populations. Two steps recommended tissue culture B. stenophylla. But it start with media containing 0.2 mg/L induction followed by sub–culturing 2 +0.4 rapid elongation. This suggests that should remain as situ seedling production under optimum nursery conditions be near natural
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