The Missing Fungi: New Insights from Culture-Independent Molecular Studies of Soil

摘要: Molecular surveys of soil fungi have received much less attention despite the fact that dominate decomposition and nutrient cycles in many soils. The ability to sequence fungal DNA from samples has opened up a new realm possibilities for identifying groups. A clone library approximates random sample soil, is likely be biased toward some groups over others based on variables each step procedure used. Assembling Fungal Tree Life (AFTOL) initiative definitively clarified phylogenetic tree all known fungi. Three rDNA regions are commonly used identify fungi: 18S small subunit (SSU); internal transcriber spacer sequences ITS-1 ITS-2; 28S large (LSU). This chapter reviews lineages been discovered various libraries past 10 years. Most date only at genus or species level. talks about two main discoveries, Chytridiomycota Ascomycota, after reviewing other important but unrelated clades also libraries. It flesh out family as quickly possible, it possible missing can go extinct before they ever existed.