PAX3 is overexpressed in human glioblastomas and critically regulates the tumorigenicity of glioma cells.

摘要: Paired box 3 (PAX3) is overexpressed in glioma tissues compared to normal brain tissues, however, the pathogenic role of PAX3 human cells remains be elucidated. In this study, we selected cell lines U251, U87, SHG-44, and astrocytes, 1800, which have differential expression depending upon person. SiRNA targeting overexpression vectors were transfected into U87 SHG-44 lines, proliferation, invasion, apoptosis, differentiation examined by CCK-8 assays, transwell chamber tunnel staining, Annexin V/PI analysis, Western blotting, respectively. addition, used subcutaneous tumor models study effect on growth vivo. We found that was upregulated three lines. knockdown inhibited proliferation induced apoptosis U87MG glioblastoma line, whereas upregulation promoted increased invasion line. Moreover, together with chemotherapeutic treatment could increase susceptibility drug. nude mice using U-87MG inhibition suppressed tumorigenicity, formation These results indicate essential for gliomagenesis; thus, or its downstream targets may lead novel therapies disease.