Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library
作者： Mark D. Adams , M. Bento Soares , Anthony R. Kerlavage , Chris Fields , J. Craig Venter
DOI: 10.1038/NG0893-373
关键词: Cloning 、 Biology 、 cDNA library 、 Expressed sequence tag 、 Gene 、 Ribosomal RNA 、 Amyloid precursor protein 、 Genetics 、 Cdna sequencing
摘要: A human infant brain cDNA library, made specifically for production of expressed sequence tags (ESTs) was evaluated by partial sequencing over 1,600 clones. Advantages this constructed EST sequencing, include the use directional cloning, size selection, very low numbers mitochondrial and ribosomal transcripts, short polyA tails, few non-recombinants a broad representation transcripts. 37% clones were identified, based on matches to 320 different genes in public databases. Of these, two proteins similar Alzheimer's disease amyloid precursor protein identified.
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