Molecular cloning and expression analysis of two distinct F-type lectins from the rock bream, Oplegnathus fasciatus.
作者： Hyung-Jun Park , Ju-Won Kim , Eun-Gyeong Kim , Ha-Na Kim , Young-Sun Chae
DOI: 10.1016/J.DCI.2011.05.011
关键词: Microbiology 、 Molecular cloning 、 Edwardsiella tarda 、 Open reading frame 、 Galectin 、 Biology 、 Oplegnathus fasciatus 、 Complementary DNA 、 Pentraxins 、 Ficolin
摘要: Abstract Several lectin families characterized by distinct signature sequence motifs and structural folds, such as C-type, peptidoglycan recognition protein, ficolin, pentraxins, most recently galectins, have been implicated in immune surveillance. In this study, two F-type lectins RbFTL-1 RbFTL-2, from the rock bream ( Oplegnathus fasciatus ), were identified their expression was analyzed. The full-length cDNA of composed 1204 bp with a 945-bp open reading frame (ORF) that encoded 314 amino-acid while RbFTL-2 consisted 1614 bp 951-bp ORF encoding 316 protein. mRNAs predominately expressed head-kidney liver, respectively. Levels mRNA transcript increased up to 5.0- 2.8-fold trunk-kidney compared muscle, respectively, those 12.0-fold liver. differentially up-regulated challenged Edwardsiella tarda , Streptococcus iniae RSIV, significant increases at 1 3 h post-challenge controls.
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