Expression Analysis of Lily Type Lectin Isotypes in the Rock Bream, Oplegnathus fasciatus: in the Tissue, Developmental Stage and Viral Infection.

摘要: Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in recognition elimination of pathogens via innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks early response fish. However, this study limited information lectin against viral infection. In present study, lectin-2 -3 Oplegnathus fasciatus (OfLTL-2 3) have been presented be included B-lectin domain two D-mannose binding sites amino acid sequence an feature for fundamental structure. To investigate functional properties OfLTLs, tissue distribution healthy rock bream temporal expression during developmental stage analysis are performed using quantitative real-time PCR. OfLTL-2 3 predominantly expressed liver skin, but rarely other organ. Also, transcripts OfLTLs not its increased after immune-related organs which fully formed. challenge experiment with RBIV (rock iridovirus), much more strongly late than early, unlike previously known. These results suggest specifically tissues those formed can inferred intensively second half virus