Reconstitution of Human Urothelium From Monolayer Cultures

摘要: AbstractPurpose: We established a 3-dimensional organ culture model of urinary tract tissue in which to study the effects seeding cultured urothelial cells onto de-epithelialized stroma.Materials and Methods: Normal human tissues were placed or used establish cell cultures. At passage 2 cultures harvested reconstitute autologous by stroma. Organ at intervals analyzed immunohistology with panel antibodies against differentiation associated antigens, cytokeratins, adhesion molecules, extracellular matrix components proliferation antigens.Results: Human maintained for least 18 weeks they retained transitional epithelial morphology expression normal situ antigenic characteristics. Within reconstitution recombined formed stratified, polarized,...