Remineralized bone matrix as a scaffold for bone tissue engineering.

摘要: There is a need for improved biomaterials use in treating non-healing bone defects. A number of natural and synthetic have been used the regeneration tissue with mixed results. One approach to modify native via decellularization or other treatment as scaffolding repair. In this study, our goal was improve on previously published alternating solution immersion (ASI) method fabricate robust, biocompatible, mechanically competent biomaterial from demineralized matrix (DBM). The includes an antigen removal (AR) step which improves mineralization stiffness while removing unwanted proteins. chemistry mineral remineralized (RBM) consistent dicalcium phosphate dihydrate (brushite), material clinically healing applications. Mass spectrometry identified proteins removed AR include α-2 HS-glycoprotein osteopontin, noncollagenous (NCPs) known inhibitors biomineralization. Additionally, RBM supported survival, proliferation, differentiation human mesenchymal stromal cells (MSCs) vitro well better than widely including DBM PLG scaffolds. DNA content increased more 10-fold compared PLG; likewise, osteogenic gene expression significantly after 1 2 weeks. We demonstrated that ASI remineralization has capacity stiff biocompatible RBM, suitable cell culture © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4480–4490, 2014.