Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro

摘要: Abstract Multipotent cell types are rapidly becoming key components in a variety of tissue engineering schemes, and mesenchymal stem cells (MSCs) emerging as an important tool bone regeneration. Although several soluble signals influencing osteogenic differentiation MSCs vitro well-characterized, relatively little is known about the influence substrate signals. This study was aimed at elucidating effects bone-like mineral (BLM), which vital process bonding to orthopedic implant materials, on human vitro. Growth BLM film (carbonate apatite, Ca/P=1.55) poly(lactide- co -glycolide) (PLG) substrates achieved via surface hydrolysis subsequent incubation modified simulated body fluid. The demonstrated significantly increased adsorption fibronectin, supported enhanced proliferation (hMSCs) relative PLG substrates. In absence supplements hMSCs did not display high expression markers or PLG. presence exhibited greater than substrates, measured by alkaline phosphatase activity osteocalcin production. Taken together, these data support concept that MSC growth differentiation, highlighting importance carrier material composition cell-based schemes.