Cyclophosphamide and prednisolone exacerbate the severity of intestinal paratuberculosis in Mycobacterium paratuberculosis monoassociated mice

摘要: In this study we examined the effects of continuous oral administration immunosuppressive agents cyclophosphamide and prednisolone on susceptibility gnotobiotic nu/+ BALB/c mice to intestinal paratuberculosis. Treatment with either or led fecal shedding Mycobacterium paratuberculosis, increased numbers M. paratuberculosis recovered from tract liver, levels similar those untreated nu/nu mice. Numerous acid-fast bacilli granulomas were observed within tracts livers treated contrast, control infrequently shed small bacilli, harbored low in their tracts, did not have visible acid fast tissues. Spleen cells mice, had a reduced ability proliferate vitro response mitogen antigens. These observations are consistent previous evidence that cellular immunity restricts development