Molecular epidemiology of rotavirus diarrhea among children and adults in Nepal: detection of G12 strains with P[6] or P[8] and a G11P[25] strain.

摘要: In anticipation of a rotavirus vaccine in Nepal, this study was undertaken to determine the distribution G and P serotypes electropherotypes rotaviruses order examine if there is any emerging serotype or unusual strain circulating children adults Nepal. Of 1,315 diarrheal stool specimens, detected by an enzyme-linked immunosorbent assay 116 (17%) 666 patients less than 5 years age, 18 (7%) 260 14 19 (5%) 358 15 age older. Approximately 75% diarrhea occurred age. 70% found each three groups belonged G1P[8]. Interestingly, were 29 (20%) G12 carrying either P[8] P[6] one (0.7%) G11 P[25] genotype. RNA polyacrylamide gel electrophoresis discriminated strains (electropherotypes), among which codominant G1P[8] long patterns. Five rotaviruses, all had The fact that 20% multiple suggest are not more rare but they pose challenge current future vaccines. presence as defined suggests richness gene pool where may continue emerge.