Abiotic metal stress enhances diosgenin yield in Dioscorea bulbifera L. cultures.

作者: Alka Narula , Sanjeev Kumar , P. S. Srivastava

DOI: 10.1007/S00299-005-0945-9

关键词: BiologyProlineMurashige and Skoog mediumDioscorea bulbiferaBotanyDioscoreaceaeAbiotic componentDiosgeninYield (chemistry)HorticultureMetal

摘要: Lower concentrations of CuSO4 (25–75 μM) in the MS medium supplemented with 0.1 mg l−1 IAA+5.0 mg Kn+500 mg CH+10 mg Cyst hyd enhanced growth regenerants Dioscorea bulbifera L. (75 μM) induced an appreciable diosgenin yield compared to those obtained on media without Cu. The presence Cu thus seems stimulate production. also differentiated bulbils lower At (100 μM), however, cultures showed poor as well a low yield. Increased proline and protein contents were recorded grown Cu-enriched media.

参考文章(36)
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
L. S. Bates, R. P. Waldren, I. D. Teare, Rapid determination of free proline for water-stress studies Plant and Soil. ,vol. 39, pp. 205- 207 ,(1973) , 10.1007/BF00018060
Georgia Ouzounidou, Copper-induced changes on growth, metal content and photosynthetic function of Alyssum montanum L. plants Environmental and Experimental Botany. ,vol. 34, pp. 165- 172 ,(1994) , 10.1016/0098-8472(94)90035-3
Gunawan Indrayanto, Lestari Rahayu, Abdul Rahman, Premita Noeraeni, Effect of Calcium, Strontium, and Magnesium Ions on the Formation of Phytosteroids in Callus Cultures of Agave amaniensis. Planta Medica. ,vol. 59, pp. 97- 98 ,(1993) , 10.1055/S-2006-959618
YUZO SHIOI, HIROKO TAMAI, TSUTOMU SASA, Inhibition of Photosystem II in the Green Alga Ankistrodesmus falcatus by Copper Physiologia Plantarum. ,vol. 44, pp. 434- 438 ,(1978) , 10.1111/J.1399-3054.1978.TB01651.X
Alia, P. Pardha Saradhi, Proline Accumulation Under Heavy Metal Stress Journal of Plant Physiology. ,vol. 138, pp. 554- 558 ,(1991) , 10.1016/S0176-1617(11)80240-3
JudithM. Furze, MichaelJ.C. Rhodes, AdrianJ. Parr, RichardJ. Robins, IanM. Withehead, DavidR. Threlfall, Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium. Plant Cell Reports. ,vol. 10, pp. 111- 114 ,(1991) , 10.1007/BF00232039
F. Quigley, Diosgenin in West African Dioscorea Plants Planta Medica. ,vol. 33, pp. 414- 415 ,(1978) , 10.1055/S-0028-1097404
Laszio Purnhauser, Gabor Gyulai, Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures Plant Cell Tissue and Organ Culture. ,vol. 35, pp. 131- 139 ,(1993) , 10.1007/BF00032962
Josef Thumann, Erwin Grill, Ernst-L. Winnacker, Meinhart H. Zenk, Reactivation of metal-requiring apoenzymes by phytochelatin—metal complexes FEBS Letters. ,vol. 284, pp. 66- 69 ,(1991) , 10.1016/0014-5793(91)80763-S