Disruption of baker's yeast by a new bead mill

作者: Murray Moo-Young , Helen Huihua Mao

DOI: 10.1007/BF00157432

关键词: Bead (woodworking)Cell disruptionCell concentrationYeastMillSuspension cultureModel systemYield (chemistry)ChromatographyChemistry

摘要: A continuous high-speed bead mill of novel design (Sulzer Annu Mill 01) was tested for cell disruption baker's yeast as a model system. The efficiency evaluated the relative amount released protein. effects rotation speed, concentration and flow rate suspension on were investigated. maximum yield protein found to be 2.62 kg protein/L.h. This appears more effective than existing commercially available mills.

参考文章(6)
David F Ollis, James Edwin Bailey, Biochemical engineering fundamentals Published in <b>1986</b> in New York by McGraw-Hill. ,(1986)
Donald Deters, Urs Müller, Henri Homberger, Breakage of yeast cells: Large scale isolation of yeast mitochondria with a continuous-flow disintegrator Analytical Biochemistry. ,vol. 70, pp. 263- 267 ,(1976) , 10.1016/S0003-2697(76)80067-X
Yusuf Chisti, Murray Moo-Young, Disruption of microbial cells for intracellular products Enzyme and Microbial Technology. ,vol. 8, pp. 194- 204 ,(1986) , 10.1016/0141-0229(86)90087-6
J. Limon-Lason, M. Hoare, C. B. Orsborn, D. J. Doyle, P. Dunnill, Reactor properties of a high‐speed bead mill for microbial cell rupture Biotechnology and Bioengineering. ,vol. 21, pp. 745- 774 ,(1979) , 10.1002/BIT.260210503
Maggie Follows, P. J. Hetherington, P. Dunnill, M. D. Lilly, Release of enzymes from bakers' yeast by disruption in an industrial homogenizer Biotechnology and Bioengineering. ,vol. 13, pp. 549- 560 ,(1971) , 10.1002/BIT.260130408
Gudmund Hedenskog, Lennart Enebo, Jitka Vendlová, Bohumír Prokeš, Investigation of some methods for increasing the digestibility in vitro of microalgae. Biotechnology and Bioengineering. ,vol. 11, pp. 37- 51 ,(1969) , 10.1002/BIT.260110104