Properties of monocyte chemotactic and activating factor (MCAF) purified from a human fibrosarcoma cell line.

摘要: A monocyte chemotactic and activating factor (MCAF) has been purified from TNF-stimulated 8387 human fibrosarcoma cell line-conditioned media. The MCAF showed microheterogeneity yielding two bands on SDS-PAGE analysis. Fibrosarcoma-derived specifically competed with THP-1 (a monocytic line)-derived 125I-labeled in binding to PBMC, whereas a similar basic heparin-binding leukocyte chemoattractant, IL-8, did not. stimulated superoxide anion N-acetyl beta-D glucosaminidase-releasing activity monocytes, as well cytostatic augmenting against tumor cells for monocytes. When injected subcutaneously into Lewis rat ears, the also induced considerable vivo local infiltration beginning at 3 h becoming maximal 18 h. In conclusion, data presented this paper indicate that is potent activator of monocytes recruitment acts through receptors are specific novel molecule. This cytokine might have an important role growth control due its ability attract activate