Oxygen Michaelis constants for tyrosinase

作者： J N Rodríguez-López , J R Ros , R Varón , F García-Cánovas

关键词: Michaelis–Menten kinetics 、 Reaction mechanism 、 Stereochemistry 、 Monophenol monooxygenase 、 Physical chemistry 、 Oxygen 、 Constant (mathematics) 、 Tyrosinase 、 Kinetic analysis 、 Chemistry

摘要: The Michaelis constant of tyrosinase for oxygen in the presence monophenols and o-diphenols, which generate a cyclizable o-quinone, has been studied. This depends on nature monophenol o-diphenol is always lower former than latter. From mechanism proposed from its kinetic analysis [Rodriguez-Lopez, J. N., Tudela, J., Varon, R., Garcia-Carmona, F. Garcia-Canovas, (1992) Biol. Chem. 267, 3801-3810] quantitative ratio established between constants their o-diphenols. used determination with when value cannot be calculated experimentally.