Sensing small neurotransmitter–enzyme interaction with nanoporous gated ion-sensitive field effect transistors

摘要: Ion-sensitive field effect transistors with gates having a high density of nanopores were fabricated and employed to sense the neurotransmitter dopamine selectivity detectability at micromolar range. The nanoporous structure was produced by applying relatively simple anodizing process, which yielded porous alumina layer pores exhibiting mean diameter ranging from 20 35 nm. Gate-source voltages demonstrated pH-dependence that linear over wide range could be understood as changes in surface charges during protonation deprotonation. large area provided allowed physical immobilization tyrosinase, is an enzyme oxidizes dopamine, on transistors, thus, acid-base behavior their surfaces. Concentration-dependent interacting immobilized tyrosinase showed dependence into physiological interest for concentration gate-source voltages. In comparison previous approaches, response time fast detecting obtained. Additionally, assays other neurotransmitters are abundantly found brain examined. These results demonstrate ion-sensitive can easily used immobilize specific readily selectively detect small molecule based its interaction receptor. Therefore, it serve technology platform molecular studies neurotransmitter-enzyme binding drugs screening.