Development of an efficient two-element transposon tagging system in Arabidopsis thaliana
作者： Ian Bancroft , AnujM. Bhatt , Christina Sjodin , Steve Scofield , JonathanD.G. Jones
DOI: 10.1007/BF00265443
关键词: Genetics 、 Biology 、 Transposon tagging 、 Hygromycin B 、 Arabidopsis thaliana 、 Transposable element 、 Open reading frame 、 Transactivation 、 Gene 、 Transposase
摘要: Modified Ac and Ds elements, in combination with dominant markers (to facilitate monitoring of excision, reinsertion segregation the elements) were introduced into Arabidopsis thaliana ecotype Landsberg erecta. The frequencies somatic germinal transactivation elements monitored using a streptomycin resistance assay. Transactivation was significantly higher from stable (sAc) carrying 537 bp deletion CpG-rich 5' untranslated leader transposase mRNA than wild-type sAc. However, substitution central 1.77 kb open reading frame (ORF) hygromycin marker did not alter excision frequency element. beta-Glucuronidase (GUS) or iaaH linked to source allow identification plants which had segregated away transposed element, eliminating possibility re-activation. Segregation marker, sAc progeny showing Ds. 29% inheriting carried
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