Heterologous transposon tagging as a tool for the isolation of plant genes

摘要: Isolation of plant genes by conventional techniques requires knowledge a gene product. In routine procedures for cloning, genomic or expression libraries are screened with probes made mRNA protein, respectively. Also, based on the cDNA protein sequence, oligonucleotides can be designed to apply PCR approach. However, unknown products alternative strategies that have genetic basis required. Recently, isolation successfully been applied starting map position gene. These map-based cloning approaches, however, don’t involve mutant phenotypes may hamper identification genes. order obtain mutants allow simultaneous corresponding genes, developed using transposons. By insertional mutagenesis obtained which responsible molecularly tagged and hence cloned.