Further investigation of lipid-substituted poly(L-Lysine) polymers for transfection of human skin fibroblasts.

摘要: Enabling gene expression in skin fibroblasts using safe, nonviral delivery has the potential to stimulate wound healing and aid tissue engineering efforts. In this study, several lipid-substituted poly(L-Lysines) (PLL) were investigated for their ability deliver a plasmid DNA (pEGFP) human fibroblasts. While native PLLs showed complete complexation with pEGFP, polymers higher lipid substitution more resilient dissociation after heparin treatment. All good protection of pEGFP against DNase I II digestion vitro. studies fluorescently labeled that PLL lacked into cells, whereas most gave efficient cells. Extent was an important factor efficiency. The intracellular intact up 7 days. An RT-PCR methodology indicated successful transcription reporter GFP gene, which not case when cells transfected blank containing no functional gene. Further flow cytometry protein obtained substituted myristic stearic acid, latter displaying relatively lower toxicity. We conclude substituting lipids on results effective carriers extent substitution, rather than individual lipid, appeared be critical delivery.