Determination of binding thermodynamics.
作者： Peter C. Dedon
DOI: 10.1002/0471142700.NC0802S00
关键词: Thermodynamics 、 Ligand (biochemistry) 、 Binding constant 、 Dissociation constant 、 Curve fitting 、 Chemistry 、 Small molecule
摘要: This unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers determination simple, apparent association/dissociation constants. The concentration DNA-bound ligand free are determined binding constant is extracted from these data. Data gathering curve fitting discussed.
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