Fluorescent zinc(II) complexes for gene delivery and simultaneous monitoring of protein expression

摘要: Two new zinc(II) complexes, [Zn(L-His)(NIP)]+(1) and [Zn(acac)2(NIP)](2) (where NIP is 2-(naphthalen-1-yl)-1H-imidazo[4,5-f][1,10]phenanthroline, acac = acetyl acetone), have been synthesized characterized by elemental analysis, UV-vis, fluorescence, IR, 1H NMR electron spray ionization mass spectroscopies. Gel retardation assay, atomic force microscopy dynamic light scattering studies show that 1 2 can induce the condensation of circular plasmid pBR322 DNA into nanometer size particles under ambient conditions. Treatment with 5 mM EDTA restored 30% supercoiled form DNA, revealing partial reversibility condensation. The in vitro transfection experiment demonstrates complexes be used to deliver pCMV-tdTomato-N1 which expresses red fluorescent protein. confocal nature advantageous for visualizing intracellular delivery metal as well efficiency using two distinct emission windows.