D-SP5 Peptide-Modified Highly Branched Polyethylenimine for Gene Therapy of Gastric Adenocarcinoma.
作者： Xue Li , Zuoxu Xie , Cao Xie , Weiyue Lu , Chunli Gao
DOI: 10.1021/ACS.BIOCONJCHEM.5B00137
关键词:
摘要: Peptide-mediated targeting of tumors has become an effective strategy for cancer therapy. Retro-inverso peptides resist protease degradation and maintain their bioactivity. We used the retro-inverso peptide D(PRPSPKMGVSVS) (D-SP5) as a ligand to develop gene therapy gastric adenocarcinoma. D-SP5 higher affinity human adenocarcinoma (SGC7901) cells compared with that its parental peptide, L(SVSVGMKPSPRP) (L-SP5). Polyethylenimine (PEI)/pDNA, polyethylene glycol (mPEG)-PEI/pDNA D-SP5-PEG-PEI/pDNA were prepared further study. Quantitative luciferase assays showed transfection efficiency D-SP5-PEG-PEI/pGL4.2 was larger mPEG-PEI/pGL4.2. Flow cytometry revealed apoptosis rates SGC7901 treated D-SP5-PEG-PEI/pTRAIL than mPEG-PEI/pTRAIL. Western blot indicated expression tumor necrosis factor-related inducing (TRAIL) protein in D-SP5-PEG-PE...
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