High‐dose pyridoxal supplemented culture medium inhibits the growth of a human malignant melanoma cell line
作者： Dennis M. DiSorbo , Larry Nathanson
DOI: 10.1080/01635588309513773
关键词: Pyridoxal kinase 、 Metabolite 、 Biology 、 Antagonist 、 Uridine 、 Pyridoxal 、 Glucose uptake 、 Melanoma 、 Thymidine 、 Biochemistry 、 Molecular biology
摘要: Abstract The in vitro growth characteristics of a human malignant melanoma cell line cultured 0.5 mM pyridoxal supplemented medium were studied. Experimentation revealed that the high‐dose severely inhibited cells over 72‐hour period. Additional experimentation showed for 6 hours took up 25% less and incorporated 20% [3H]uridine than control cultures. [3H]Glucose uptake was reduced by 23% at this time point. [3H] Thymidine 12%, but no inhibition detected [3H]thymidine incorporation. When vitamin B6 antagonist 4‐deoxypyridoxine (which competes with kinase) added to medium, incorporation from 19% 6%. However, did not reverse uptake. These results indicate its metabolite, 5'‐...
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