Sequence and Comparative Analysis of the Mouse 1-Megabase Region Orthologous to the Human 11p15 Imprinted Domain

摘要: A major barrier to conceptual advances in understanding the mechanisms and regulation of imprinting a genomic region is our relatively poor overall organization genes potentially important cis-acting regulatory sequences that lie nonexonic segments make up 97% genome. Interspecies sequence comparison offers an effective approach identify from conserved functional elements. In this article we describe successful use comparing approximately 1-Mb imprinted domain on mouse chromosome 7 its orthologous human 11p15.5. Within region, identified 112 exons known as well novel gene uniquely termed Msuit, was found be imprinted. addition these coding elements, 33 CpG islands 49 nonexonic, nonisland met criteria being conserved, making 4.1% total sequence. These noncoding elements were generally clustered near majority between Igf2 H19 or within Kvlqt1. Finally, location provided evidence suggested two-island rule for genes. This study provides first global view architecture entire candidate subsequent analyses.