Analysis of the Quality and Utility of Random Shotgun Sequencing at Low Redundancies

摘要: The currently favored approach for sequencing the human genome involves selecting representative large-insert clones (100–200 kb), randomly shearing this DNA to construct shotgun libraries, and then many different isolates from library. This method, entitled directed random sequencing, requires highly redundant obtain a complete accurate finished consensus sequence. Recently it has been suggested that rapidly generated lower redundancy sequence might be of use scientific community. Low-redundancy examined previously using simulated data sets. Here we utilize trace number projects submitted GenBank perform reconstruction experiments mimic low-redundancy sequencing. These sequences have completeness quality product, information content, usefulness interspecies comparisons.The presented here suggest three strategies, each with utilities. (1) Nearly can obtained by library at sixfold redundancy. may therefore represent good point switch approach. (2) Sequencing performed as little twofold find most about exons, EST hits, putative exon similarity matches. (3) To contiguity coding regions, three- fourfold would appropriate. From these results, useful intermediate product Such allow large amount biologically extracted while postponing majority work involved in producing high