Differential Expression of miRNAs in Hypoxia ("HypoxamiRs") in Three Canine High-Grade Glioma Cell Lines.

摘要: Dogs with spontaneous high-grade gliomas increasingly are being proposed as useful large animal pre-clinical models for the human disease. Hypoxia is a critical microenvironmental condition that common in both canine and drives increased angiogenesis, chemo- radioresistance, acquisition of stem-like phenotype. Some this effect mediated by hypoxia-induced expression microRNAs, small (~22 nucleotides long), non-coding RNAs can modulate gene through interference mRNA translation. Using an vitro model three glioma cell lines (J3T, SDT3G, G06A) exposed to 72 h 1.5% oxygen vs. standard 20% oxygen, we examined global "hypoxamiR" profile using RNA-Seq performed pathway analysis targeted genes Panther NetworkAnalyst. Important pathways include many well-established important biology, general cancer hypoxia, immunology, stem-ness, among others. This work provides first examination hypoxia on miRNA context glioma, highlights similarities