In vivo analysis of chromatin structure.
作者： Kenneth S. Zaret
DOI: 10.1016/S0076-6879(99)04036-7
关键词: Computational biology 、 Function (biology) 、 Deoxyribonuclease I 、 Genetics 、 In vivo 、 Biology 、 Nucleosome 、 Nucleus 、 Cell nucleus 、 DNA 、 Chromatin
摘要: Publisher Summary Chromatin structure in vivo covers a wide range of cell biological and biochemical perspectives on the physical state genes nucleus. The chapter describes several techniques used to identify chromatin changes or situ . An analysis can provide information regarding gene function. discusses that is found within intact cells, permeabilized isolated nuclei. Three parameters govern ability obtain useful from experiment: quality substrate, structural specificity probe, sensitivity an assay. Each these discussed separately terms benefits hazards relevant techniques. A few references are given for each method; either clear generally accepted description experimental details illustrate novel ways which be used.
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