CYP1A Expression in Caged Rainbow Trout Discriminates Among Sites with Various Degrees of Polychlorinated Biphenyl Contamination

摘要: It has become increasingly apparent that resident fish can develop resistance to chemicals in their environment, thus compromising usefulness as sentinels of site-specific pollution. By using a stream system whose appear have developed pollutant (Brammell et al., Mar Environ Res 58:251–255, 2005), we tested the hypothesis pollutant-inducible biomarker, cytochrome P4501A (CYP1A), measured field-caged juvenile rainbow trout (Oncorhynchus mykiss), would reflect relative pollution differences between reference and polychlorinated biphenyl (PCB)-contaminated sites. Trout were caged Town Branch/Mud River (Logan County, KY), undergoing remediation for PCBs. Fish held remediated (Town Branch), unremeditated (Mud River), sites 2 weeks during spring 2002. At end this period, gill hepatic CYP1A expression measured. To evaluate PCB exposure provide point against which calibrate response, levels quantified sediments from each site. Hepatic clearly detected presence PCBs system. Sediment produced identical rankings study Gill expression, although suggestive site differences, was not statistically different among Unlike fish, failed show waterway al. proved be sensitive discriminator contamination In summary, determined reflected situ exposure. The paradigm confirmed sufficient caging period evaluating response species at these temperatures (13–19°C). addition, studies demonstrate tissue-specific insights into likely routes We conclude is reliable inexpensive first-pass determination environmental bioavailability aqueous systems.