Unusual Isothermal Hysteresis in DNA i-Motif pH Transitions: A Study of the RAD17 Promoter Sequence.
作者： R. Aaron Rogers , Aaron M. Fleming , Cynthia J. Burrows
DOI: 10.1016/J.BPJ.2018.03.012
关键词: Gene 、 Isothermal process 、 Mutant 、 Chemistry 、 Biophysics 、 Titration 、 Titration curve 、 Nucleic Acid Denaturation 、 DNA 、 Footprinting
摘要: We have interrogated the isothermal folding behavior of DNA i-motif human telomere, dC19, and a high-stability i-motif-forming sequence in promoter repair gene RAD17 using physiological solution temperature conditions. developed circular-dichroism-spectroscopy-based pH titration method that is followed by analysis curves derivative domain found observed pH-dependent can be significantly different and, some cases, multiphasic, with dependence on how rapidly induced. Interestingly, telomere exhibits unusual hysteresis which unfolding process always occurs at higher than the folding process. For i-motif, rapid injection into low-pH results triphasic completely diminished when samples are slowly folded stepwise manner via titration. Chemical footprinting the RAD17 titrations dT-substituted mutants were used to develop model of RAD17 unfolding. These may provide valuable information pertinent use sensors materials, as well as insight potential biological activity sequences under or instantaneous changes pH.
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