Determination of the conjugated linoleic acid-containing triacylglycerols in New Zealand bovine milk fat

摘要: Reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection at 233 nm was used to separate, quantify, and identify the triacylglycerols (TAG) of milk fat that contain conjugated linoleic acid (CLA). The absorbance substantially due CLA-TAG (chromatography some representative TAG devoid CLA, such as tripalmitin triolein, showed poor responses nm, 1/800th CLA-TAG). A CLA molar extinction coefficient 23 360 L mol−1 cm−1 an HPLC UV response factor were obtained from a commercially available cis-9, trans-11-CLA standard. This only 86% reported literature values. Summation all chromatographic peaks absorbing using corrected gave good agreement independent determinations total by gas spectrophotometry. allowed quantification individual in separation typical New Zealand bovine fat. Three CLA-containing TAG, CLA-dipalmitin, CLA-oleoyl-palmitin CLA-diolein, prepared interesterification respective fatty methyl esters assign reversed-phase fat, which coincident largest peak. Band fractions argentation thin-layer similarly employed five predominant groups fat: CLA-disaturates, CLA-oleoyl-saturates, CLA-vaccenyl-saturates, CLA-vaccenyl-olein, CLA-diolein.