Real-time electrical detection of the formation and destruction of lipid bilayers on silicon nanowire devices

摘要: Abstract Silicon nanowire (Si NW) two-terminal devices were fabricated to electrically probe the real-time formation and destruction of lipid bilayers. A liposome solution, containing same ratio zwitterionic/anionic lipids that are present in an Escherichia coli cell membrane, was applied NW devices. Lipid bilayer on Si NWs detected in-situ by observing electrical resistance changes complemented confocal fluorescence microscopy imaging. The bilayers resulted a 1% 2% decrease device current, consistent with negative gating effect surface. demonstrated a ≈ 1 min response time encapsulation. Removal layer achieved exposing detergent, which conductance returning its original value a ≈ 2 min recovery time. coated demonstrate novel platform enable probing bacterial membrane mechanisms, interactions, reactions.