Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
作者： Ophir Shalem , Neville E. Sanjana , Ella Hartenian , Xi Shi , David A. Scott
关键词: CRISPR 、 Vemurafenib 、 Genomic library 、 Gene 、 Cas9 、 Gene Knockout Techniques 、 Induced pluripotent stem cell 、 Guide RNA 、 Genetics 、 Biology
摘要: The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic repeats)–associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells. First, we used the GeCKO library to identify genes essential for …
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