Cas9-FokI fusion proteins and uses thereof

摘要: Some aspects of this disclosure provide compositions, methods, and kits for improving the specificity RNA-programmable endonucleases, such as Cas9. Also provided are variants Cas9, e.g., Cas9 dimers fusion proteins, engineered to have improved cleaving nucleic acid targets. site-specific modification using proteins (e.g., nuclease-inactivated fused a nuclease catalytic domain). Such useful in clinical research settings involving DNA, example, genomic modifications.