Programmable Modification of DNA

摘要: A self-reconfiguring genome uses a cassette having operons or DNA sequences that code for guide RNA, reverse transcriptase, donor and CRISPR cleavage enzyme. may be based on lambda recombineering of in situ generated oligonucleotides. method programmable self-modification cellular includes transcribing RNA from cassette, associating the transcribed guideRNA with enzyme, intercalcating region complimentary sequence within an integration site genome, cutting upstream PAM site; donorRNA, translating donorRNA to double-stranded DNA, recombining via homologous recombination at cut site. set cascadable multiplexable genetic logic gates universal input/output single-strand annealing non-homologous end joining, comprises transcription promoters terminators, regions, sequences, enzymes system.