Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine
作者： A. Elif Böyükbayram , Senem Kıralp , Levent Toppare , Yusuf Yağcı
DOI: 10.1016/J.BIOELECHEM.2006.01.006
关键词: Polyphenol oxidase 、 Catechol oxidase 、 Nuclear chemistry 、 Fourier transform infrared spectroscopy 、 Chemistry 、 Immobilized enzyme 、 Electrochemistry 、 Organic chemistry 、 Supporting electrolyte 、 Thiophene 、 Polytetrahydrofuran
摘要: Electrochemically produced graft copolymers of thiophene capped polytetrahydofuran (TPTHF1 and TPTHF2) pyrrole were achieved by constant potential electrolysis using sodium dodecylsulfate (SDS) as the supporting electrolyte. Characterizations based on Fourier transform infrared spectroscopy (FTIR) scanning electron microscopy (SEM). Electrical conductivities measured four-probe technique. Novel biosensors for phenolic compounds constructed immobilizing polyphenol oxidase (PPO) into conducting prepared electropolymerization with polytetrahydrofuran. Kinetic parameters, maximum reaction rate (V(max)) Michaelis-Menten (K(m)) optimum conditions regarding temperature pH determined immobilized enzyme. Operational stability shelf-life enzyme electrodes investigated. Enzyme used to determine amount in two brands Turkish red wines found very useful owing their high kinetic parameters wide working range.
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