Engineering the fatty acid synthesis pathway in Synechococcus elongatus PCC 7942 improves omega-3 fatty acid production

摘要: The microbial production of fatty acids has received great attention in the last few years as feedstock for renewable energy. main advantage using cyanobacteria over other organisms is their ability to capture energy from sunlight and transform CO2 into products interest by photosynthesis, such acids. Fatty acid synthesis a ubiquitous well-characterized pathway most bacteria. However, activity enzymes involved this remains poorly explored. To characterize function some saturated cyanobacteria, we genetically engineered Synechococcus elongatus PCC 7942 overexpressing or deleting genes encoding synthase system tested lipid profile mutants. These modifications were turn used improve alpha-linolenic cyanobacterium. mutant resulting fabF overexpression fadD deletion, combined with desA desB desaturase sp. 7002, produced highest levels omega-3 acid. composition S. can be significantly modified engineering expression coding first reactions pathway. Variations mutants did not follow pattern observed Escherichia coli derivatives. Some these production. This work provides new insights strategies that might manipulate content cyanobacteria.