Direct anti-inflammatory effects of granulocyte colony-stimulating factor (G-CSF) on activation and functional properties of human T cell subpopulations in vitro.
作者： Vladimir Vladimirovich Malashchenko , Maxsim Evgenievich Meniailo , Viacheslav Anatolievich Shmarov , Natalia Dinislamovna Gazatova , Olga Borisovna Melashchenko
DOI: 10.1016/J.CELLIMM.2018.01.007
关键词: Cytokine 、 Molecular biology 、 CD38 、 CD28 、 Acquired immune system 、 T cell 、 IL-2 receptor 、 Naive T cell 、 CD3 、 Biology
摘要: Abstract We investigated the direct effects of human granulocyte colony-stimulating factor (G-CSF) on functionality T-cell subsets. CD3+ T-lymphocytes were isolated from blood healthy donors by positive magnetic separation. T cell activation with particles conjugated antibodies (Abs) to CD3, CD28 and CD2 molecules increased proportion cells expressing G-CSF receptor (G-CSFR, CD114) in all subpopulations studied (CD45RA+/CD197+ naive cells, CD45RA−/CD197+ central memory CD45RA−/CD197− effector CD45RA+/CD197− terminally differentiated cells). Upon vitro, (10.0 ng/ml) significantly specifically enhanced CD114+ CD4+ compartment. A dilution series (range, 0.1–10.0 ng/ml) was tested, no effect expression CD25 (interleukin-2 α-chain) activated cells. Meanwhile, treatment CD38+ cell, subsets, as well CD4− did not affect IL-2 production cells; relatively low concentrations down-regulated INF-γ production, while high this cytokine up-regulated IL-4 The data obtained suggests that could play a significant role both preventing development excessive potentially damaging inflammatory reactivity, constraining expansion cytodestructive
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