Modifying Dielectrophoretic Response of Nonviable Yeast Cells by Ionic Surfactant Treatment

摘要: Nonviable cells are essential biosystems, due to the functionalities they offer and their effects on viable cells. Therefore, separation immobilization of nonviable separately or in vicinity is great importance for many fundamentals investigations cell biology. However, most become less polarizable than surrounding medium at conductivities above 0.01 S/m. This means that such a medium, dielectrophoresis, despite its versatilities manipulation cells, cannot be employed immobilizing Here, we present novel approach change dielectrophoretic (DEP) response yeast by treating them with low concentrations ionic surfactants as sodium dodecyl sulfate. After this treatment, exhibit strong positive DEP response, even high conductivities. The capability treatment demonstrated two proof-of-concept experiments. First, show sorting along consecutive microelectrode arrays. Second, demonstrate each other same array. proposed technique allows platforms utilized subsequent postanalysis both without presence other.