Dielectrophoretic isolation of DNA and nanoparticles from blood.

摘要: The ability to effectively detect disease-related DNA biomarkers and drug delivery nanoparticles directly in blood is a major challenge for viable diagnostics therapy monitoring. A DEP method has been developed which allows the rapid isolation, concentration detection of from human rat whole blood. Using microarray device operating at 20 V peak-to-peak 10 kHz, wide range high molecular weight (HMW)-DNA were concentrated into high-field regions by positive DEP, while cells low-field negative DEP. simple fluidic wash removes remain where they can be detected fluorescence. HMW-DNA could 260 ng/mL, level suitable analysis cell-free circulating biomarkers. Fluorescent 40 nm 9.5 × 10(9) particles/mL, monitoring nanoparticles. rapidly isolate undiluted will benefit many diagnostic applications significantly reducing sample preparation time complexity.