Gains and losses of DNA sequences in osteosarcomas by comparative genomic hybridization

摘要: Our aim was to identify chromosomal regions that are likely harbor previously unknown genes with an important role in the genesis of osteosarcoma. Comparative genomic hybridization used screen for losses and gains DNA sequences along all chromosome arms 11 tumors. Extensive genetic aberrations, average changes/tumor (range, 1-20), were found 10 specimens. High level amplifications small detected eight These involved 12q12-q13 region (known contain SAS-MDM2 locus) several unreported amplification sites such as 17p11-p12, 3q26, Xq12. When sequence evaluated, at 8q Xp most common (45%). The seen 2q, 6q, 8p, 10p (36%). In conclusion, despite very complex pattern changes osteosarcomas, certain appear be affected more often than others. Most these have not been reported implicated osteosarcomas may thus highlight locations novel development progression